Single-molecule fluorescence detection: autocorrelation criterion and experimental realization with phycoerythrin.
نویسندگان
چکیده
منابع مشابه
Single-molecule fluorescence detection: autocorrelation criterion and experimental realization with phycoerythrin.
A theory for single-molecule fluorescence detection is developed and then used to analyze data from subpicomolar solutions of B-phycoerythrin (PE). The distribution of detected counts is the convolution of a Poissonian continuous background with bursts arising from the passage of individual fluorophores through the focused laser beam. The autocorrelation function reveals single-molecule events ...
متن کاملSingle molecule probing by fluorescence and force detection.
The field of single molecule biophysics has rapidly grown in diverse directions. Enabled by improved microscopes and cameras, the temporal and spatial resolution of molecular detection has reached sub-nanometer and microsecond levels. An exciting new trend involves developing combination of fluorescence and force microscopy. Such tools allow for maneuvering and capturing extremely fine movement...
متن کاملSnapShot: Single-Molecule Fluorescence
Common uorescent agents include organic uorophores (e.g., Cy5), uorescent proteins (e.g., GFP), and semiconductor quantum dots each with different size, photobleaching, blinking, and labeling ef ciency properties. Wideeld detection with EMCCD camera enables parallel measurements. The temporal resolution is >ms, and the working concentration is limited to nM or less. Confocal detection from an e...
متن کاملExploring single-molecule dynamics with fluorescence nanoscopy
The study of molecular dynamics at the single-molecule level with fluorescence correlation spectroscopy (FCS) and far-field optics has contributed greatly to the functional understanding of complex systems. Unfortunately, such studies are restricted to length scales of >200 nm because diffraction does not allow further reduction of the measurement volume. This sets an upper limit on the applica...
متن کاملSingle-Molecule Imaging With One Color Fluorescence.
Single-molecule fluorescence imaging is a powerful tool that enables real-time observation of DNA-protein or RNA-protein interactions with a nanometer precision. Here, we provide a detailed procedure for a previously developed single-molecule fluorescence method, termed "single-molecule protein-induced fluorescence enhancement" (smPIFE). While smFRET (Förster resonance energy transfer) requires...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1989
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.86.11.4087